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prolong gold antifade medium p36930  (Thermo Fisher)


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    Thermo Fisher prolong gold antifade medium p36930
    Prolong Gold Antifade Medium P36930, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prolong+gold+antifade+medium/prolong+diamond+antifade+mountant/bio_rxiv__2025__07__16__665215-247-17-22
    Average 90 stars, based on 1 article reviews
    prolong gold antifade medium p36930 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: SMAD2 linker phosphorylation impacts overall survival, proliferation, TGFβ1-dependent gene expression and pluripotency-related proteins in NSCLC.
    Article Snippet: .. Nuclei were stained using spectral DAPI for 5 min. followed by washing with deionised water and mounting with coverslips using Prolong Gold antifade medium (Thermo Fisher Scientific). .. For mIF staining, the following antibodies were used at designated positions of the mIF cycle, primary antibody dilution and OPAL-TSA combinations: 1. panCK (clone AE1/AE3, 1–300, Zytomed Systems, Berlin, Germany; OPAL 690, 1–150, Akoya Biosciences), 2.

    Saline:

    Article Title: Endothelial c-Src Mediates Neovascular Tuft Formation in Oxygen-Induced Retinopathy.
    Article Snippet: .. After five washes in phosphate-buffered saline, retinas were post-fixed in 4% paraformaldehyde for 3 minutes at room temperature before mounting in Prolong Gold antifade medium (Invitrogen). .. Images were acquired using a Zeiss QLSM710 and Zeiss LSM880 confocal microscope.

    Labeling:

    Article Title: Zfp106 binds to G-quadruplex RNAs and inhibits RAN translation and formation of RNA foci caused by G4C2 repeats.
    Article Snippet: Stellaris RNA FISH and wash buffers were purchased from Biosearch Technologies and used according to the manufacturer’s recommendations. .. After labeling, samples were mounted in Prolong Gold antifade medium (Thermo Scientific, Inc.) and imaged using confocal microscopy as described above. ..

    Confocal Microscopy:

    Article Title: Zfp106 binds to G-quadruplex RNAs and inhibits RAN translation and formation of RNA foci caused by G4C2 repeats.
    Article Snippet: Stellaris RNA FISH and wash buffers were purchased from Biosearch Technologies and used according to the manufacturer’s recommendations. .. After labeling, samples were mounted in Prolong Gold antifade medium (Thermo Scientific, Inc.) and imaged using confocal microscopy as described above. ..



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    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), <t>DAPI</t> + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .
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    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), <t>DAPI</t> + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .
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    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), <t>DAPI</t> + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .
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    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), <t>DAPI</t> + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .
    Antifade Medium Prolong Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Image Search Results


    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4 and PS19/E3 mice. a and d, Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + cells and GFAP + cells in PS19/E4 mice. Quantified data in b,c,e,k,l are represented as mean ± s.e.m., unpaired two-sided t-test. For all experiments, PS19/E4, n = 35; and PS19/E3 n = 42. Pearson’s correlation analysis (two-sided) used for f-i .

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques:

    a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of BODIPY + neutral lipids and GFAP + astrocyte in the hippocampal DG of PS19/E4 and PS19/E3 mice at 10 months of age. b-e, Correlations between BODIPY + neutral lipids in DG (% area) and Iba1 + % area ( n = 42) ( b ), AT8 + % area ( n = 31) ( c ), NeuN + % area ( n = 39) ( d ), and hippocampal volume ( n = 39) ( e ) in PS19/E3 mice, assessed via Pearson’s correlation analysis (two-sided). f, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of PS19/E4 mice at 4.0, 6.2, 7.8, and 10.0 months of age. Scale bars, 25μm. g, Correlation between age (in months) and BODIPY + neutral lipids in the hippocampal DG of PS19/E4 mice ( n = 20). Nonlinear regression R 2 . h-j, Representative immunofluorescent images of Rab5 + early endosomes (red, h ), Rab7 + late endosomes (red, i ), and LAMP1 + lysosomes (red, j ) with Iba1 + microglia (blue) and BODIPY + neutral lipid (green) in PS19/E4 mice. All scale bars, 40μm. k, Quantification of BODIPY + marker inside LAMP1 + stain ( n = 15).

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques: Marker, Staining

    a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

    Journal: bioRxiv

    Article Title: Neuronal APOE4 drives damaging lipid accumulation via contact-dependent neuron-oligodendrocyte-microglia interaction in Alzheimer’s disease

    doi: 10.64898/2025.12.04.692390

    Figure Lengend Snippet: a, Representative immunofluorescent images of PLIN2 + lipid droplets (green), NeuN + neurons (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. b, Quantification of percent PLIN2 + area coverage in NeuN + neurons. c, Quantification of percent PLIN2 + area coverage in Iba1 + microglia. d, Representative immunofluorescent images of BODIPY + neutral lipids (green), DAPI + nuclei (white), and Iba1 + microglia (red) in the hippocampal DG of 10-month-old PS19/E4, PS19/E4/Syn1-Cre, and PS19/NSE-E4 mice. Scale bars, 40μm. e, Quantification of percent BODIPY + area coverage in the hippocampal DG mask. Quantified data in b-e represented as mean ± s.e.m., unpaired two-sided t-test. f-i, Correlations between BODIPY + % area in DG and Iba1 + % area ( f ), AT8 + % area ( g ), NeuN + % area ( h ), and hippocampal volume ( i ) in PS19/NSE-E4 mice. j, Representative immunofluorescent images of BODIPY-C11 + peroxidized lipids (green), BODIPY-C11 + non-peroxidized lipids (red), Iba1 + microglia (blue), and GFAP + astrocytes (white) in PS19/E4 mice. Scale bars, 20μm. k, Quantifications of BODIPY-C11 + peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. l, Quantifications of BODIPY-C11 + non-peroxidized lipids in Iba1 + microglia and GFAP + astrocytes in PS19/NSE-E4 mice. Quantified data in k and l are represented as mean ± s.e.m., one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparisons test. For all experiments, PS19/E4, n = 35; PS19/E3 n = 42; PS19/E4/Syn1-Cre n = 29; and PS19/NSE-E4 n = 18. Pearson’s correlation analysis (two-sided) used for f-i .

    Article Snippet: Coverslips were mounted to microscope slides with VECTASHIELD Prolong Gold with DAPI (H-1200-10, Vectors Labs) or without DAPI if the 405 channel was occupies (P36930, Vector Labs).

    Techniques: